The G94P H4 is compatible with the formation of an Asf1 H3 H4 complex

specific CD8 T effector cells might be monitored by staining using a mAb recognizing TCR V seventeen, the predominant V sequence used by these cells, The percentages of MP. 58 66 specific V 17 CD8 T cells in chA6 anergized and control cultures were similar, indicating that MP. specific CD8 T-Cells were not removed during activation while in the presence of chA6 mAb but instead became GM6001 functionally inac tivated. We next examined whether MP. 'specific CD8 T cells generated in the presence of chA6 mAb have suppressive activity. MP. 'specific effector CD8 T cells were rechallenged with APC, pulsed with MP. Inside the presence of growing variety of MLPchA6 tissue. MLPchA6 cells inhibited IFN production by MLP specific effector CD8 T cells in a dose-dependent manner, The proportions of MP. In addi tion, the decreased fraction of MP. Specific CD8 T cells expressing CD69 in nationalities supports in Organism conclusion that antigen specific CD8 T cells made,having chA6 mAb remain functionally inactivated. Equally MLP and MLPchA6 cultures expressed comparable levels of CD28, excluding the possibility that MP. specific CD8 T reg cells generated inside the presence of chA6 mAb contained CD8 CD28 suppressor T cells. The entire cytokine levels created after antigen specific activation by MP. ChA6 mAb extends human islet allograft survival in NODSCID mice To determine whether immunomodu latory effects are also exerted by chA6 mAb in vivo, we established a revised model of hu man islet transplantation in NODSCID mice. Human islets were transplanted under the renal capsule of NODSCID mice made diabetic by way of a single injection of streptozotocin. NODSCID recipient mice were injected intraperitoneally with newly isolated allogeneic PBMCs. Hu PBL NOD SCID recipient mice were treated with chA6 mAb at 1 mg kg subcutaneously at times 5 after transplantation. Normal NODSCID mice 3-Deazaneplanocin A transplanted with human islets re mained normoglycemic around 100 d after transplantation, while the mean rejection period of hu PBL NODSCID mice transplanted with human islets was 13 d.